Plasmid_Backbone
Part:BBa_K911006
Designed by: Thomas Whittaker Group: iGEM12_Cambridge (2012-09-23)
Non-standard backbone for luciferase construct
This is the sequence of the non-standard backbone that our luciferase construct, BBa_k911004, was synthesised in. It could not be assembled in the high copy number standard psb1c3 backbone due to toxicity issues. Attempts to assemble the 9kb insert into psb3c5, a low copy number biobrick plasmid have so far been unsuccessful.
The sequence of the low copy number custom backbone is provided here as a reference.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal XbaI site found at 2619 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2650
Illegal NheI site found at 580
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2656 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2650 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2650
Illegal suffix found at 2
Illegal XbaI site found at 47
Illegal XbaI site found at 2619 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2650
Plasmid lacks a suffix.
Illegal XbaI site found at 47
Illegal XbaI site found at 2619
Illegal XbaI site found at 2665
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 666
Illegal AgeI site found at 990 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
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