Plasmid_Backbone

Part:BBa_K911006

Designed by: Thomas Whittaker   Group: iGEM12_Cambridge   (2012-09-23)


Non-standard backbone for luciferase construct

This is the sequence of the non-standard backbone that our luciferase construct, BBa_k911004, was synthesised in. It could not be assembled in the high copy number standard psb1c3 backbone due to toxicity issues. Attempts to assemble the 9kb insert into psb3c5, a low copy number biobrick plasmid have so far been unsuccessful.

The sequence of the low copy number custom backbone is provided here as a reference.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 2619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal NheI site found at 580
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2650
    Illegal suffix found at 2
    Illegal XbaI site found at 47
    Illegal XbaI site found at 2619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2650
    Plasmid lacks a suffix.
    Illegal XbaI site found at 47
    Illegal XbaI site found at 2619
    Illegal XbaI site found at 2665
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 666
    Illegal AgeI site found at 990
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
None